H6409

Sigma

 

Monoclonal Anti-phospho-Histone H3 (pSer10) antibody produced in mouse

~2 mg/mL, clone H3-P, purified immunoglobulin, buffered aqueous solution

Synonym:Anti-Histone H3, phosphorylated (phosphoserine 10)
MDL number:MFCD01633984

Description

Immunogensynthetic peptide corresponding to amino acids 1-18 (pSer10) of human histone H3.
SpecificityThe antibody reacts with human histone H3, phosphorylated near its N-terminus (at Ser10), and does not detect the non-phos­phory­­lated epitope.
Physical formSolution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formpurified immunoglobulin
cloneH3-P (monoclonal)
formbuffered aqueous solution
mol wtantigen mol wt 17 kDa
species reactivityhuman
concentration~2 mg/mL
application(s)flow cytometry: 100-300 μg/mL using cultured human acute T cell leukemia Jurkat cells
 immunoblotting: suitable
 immunocytochemistry: suitable
 indirect ELISA: suitable
 microarray: suitable
isotypeIgG2a
Gene Informationhuman ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

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References

referenceJuan, G., et al., Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 32, 71-77, (1998) Abstract
 Juan, G., et al., Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp. Cell Res. 246, 212-220, (1999) Abstract
 Azuma, A., et al., Molecular basis for G2 arrest induced by 2′-C-Cyano-2′-deoxy-1-β-D-arabino-pentofuranosylcytosine and consequences of checkpoint abrogation. Cancer Res. 65, 6874-6881, (2005) Abstract